Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells. In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release. Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.
|4° C, store in dark, DO NOT FREEZE!|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
For Research Use Only.
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