BioVision Inc Lysozyme Activity Assay Kit (Fluorometric): Simple, higly-sensitive & convenient assay to measure lysozyme activity in cell & tissue lysate, nuclear extract, lysosome, purified protein samples. Detection limit: 2 µU of purified enzyme.
Manufacturer: BioVision Inc K236100
Lysozyme Activity Assay Kit (Fluorometric): Lysozyme (EC 22.214.171.124), also known as muramidase or N-acetylmuramide glycanhydrolase, is a hydrolase acting on glycosidic bonds. It hydrolyzes the ?-(1-4)-glucosidic linkage between N-acetyl-muraminic acid and N-acetyl-D-glucosamine residues present in the mucopolysaccharide cell wall. Lysozyme is ubiquitously found in a wide range of biological fluids such as tears, saliva and tissues. Elevated concentrations of lysozyme in urine and serum have been reported in patients suffering leukemia, tuberculosis, megaloblastic anemias, acute bacterial infections, ulcerative colitis, severe renal insufficiency, and nephritis. BioVision's lysozyme activity assay kit utilizes the ability of lysozyme to cleave a synthetic substrate to release the free fluorophore which can be easily quantified (Ex/Em= 360/445 nm). This kit provides a simple, ultra-sensitive assay that can detect as low as 2 ¿U/ml of Lysozyme activity in a variety of biological samples.
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