Invitrogen eBioscience MagniSort Mouse CD49b Positive Selection Kit
Designed for the magnetic separation of CD49b+ cells by positive selection. It has been optimized for the isolation of CD49b+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD49b antibody and streptavidin-coated magnetic beads.
Manufacturer: Invitrogen 8802686274
- CD49b+ cells are bound by antibody and then magnetic beads
- When placed in a magnetic field, the undesired cells can be separated from CD49b+ cells by decanting
- After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD49b (integrin alpha 2), clone DX5, and Anti-Mouse NK1.1, clone PK136. For mouse strains that do not express NK1.1 antigen such as BALB/c, SJL, AKR, CBA C3H, anti-Mouse CD335 (NKp46), clone 29A1.4, may be used
- For higher purity of CD49b+ cells, 10 µL/test of beads may be used, instead of 20 µL as indicated in the protocol, however, this may result in decreased recovery rate of CD49b+ cells
|Magnetic Cell Separation|
|Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|2° to 8°C. Do not freeze.|
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