Abnova™ Human MAP3K7 (NP_663304.1, 1 a.a. - 303 a.a.) and TAB1 (NP_006107.1, 437 a.a. - 504 a.a.) Full-length Recombinant Protein with His tag expressed in baculovirus infected Sf21 cells

Used for Func, SDS-PAGE

Manufacturer:  Abnova™ P5646

Catalog No. 89-951-659

Description

The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Specifications

• Accession Number: NP_663304.1 (Gene ID : 6885);NP_006107.1 (Gene ID : 10454)
• For Use With (Application): Functional Study, Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis
• Formulation: Liquid
• Molecular Weight: 45kDa
• Preparation Method: Baculovirus infected insect cell (Sf21) expression system
• Purity: 96% by SDS-PAGE/CBB staining
• Quantity: 5 ug
• Regulatory Status: RUO
• Common Name: MAP3K7
• Biological Activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG). Substrate: MAP2K7 [inactive mutant]. ATP: 100 uM.
• Recombinant: Yes

• Expression System: Baculovirus infected insect cell (Sf21) expression system
• Format: Liquid
• Gene ID (Entrez): 6885|10454
• Name: MAP3K7/TAB1 (Human) Recombinant Protein
• Purification Method: Ni-NTA affinity chromatography
• Quality Control Testing: Loading 1 ug protein in SDS-PAGE
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Gene Alias: TAK1|TGF1a
• Gene Symbol: MAP3K7
• Host Species: Insect
• Protein Tag: His