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Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix

Provide one-tube master mix for convenient and consistent cDNA synthesis for two-step RT-qPCR applications. The format helps streamline the RT-qPCR workflow, saves time, reduces pipeting errors to give customers sensitive, reliable and fast cDNA synthesis.

Manufacturer:  Thermo Scientific™ M1662

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Catalog No. FERM1662

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Thermo Scientific Maxima H Minus Reverse Transcriptase (RT), an engineered RNase H minus enzyme, is formulated into convenient one-tube master mix with a simplified protocol, enabling consistency and maximum control of RT-qPCR. It was developed through molecular evolution to improve thermostability, robustness and synthesis rates. Maxima H Minus RT also shows up to a 50x increase in processivity, has no RNase H activity, and demonstrates higher sensitivity.
  • Efficiently transcribes large and small amounts of RNA
  • Quick and convenient setup eliminates pipeting caused variability
  • Reduces risk of cross-contamination between samples
  • Consistently higher sensitivity for wide variety of gene targets
  • High linearity in cDNA synthesis over a broad dynamic range
  • Complete enzyme activity from 42° to 55°C


• 1 x 800 μL Maxima H Minus cDNA Synthesis Master Mix
• 1 x 200 μL Maxima H Minus cDNA Synthesis Master Mix No RT Control
• 1 x 1.25 mL nuclease-free water

Store at -5 to -30°C.

cDNA (First-Strand)
200 Reactions
Master Mix
800 μL
50°C inculbation temperature for RT reactions
cDNA Synthesis Master Mix
Dry Ice
Real-Time PCR
Reverse Transcription
Up to 20 kb
Reverse Transcription
H Minus cDNA Synthesis Master Mix Kit
Maxima H Minus
200 Reactions
Product Certifications


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