Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix

Provide one-tube master mix for convenient and consistent cDNA synthesis for two-step RT-qPCR applications. The format helps streamline the RT-qPCR workflow, saves time, reduces pipeting errors to give customers sensitive, reliable and fast cDNA synthesis.

Manufacturer:  Thermo Scientific™ M1661

Catalog No. FERM1661

Description

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT), an engineered RNase H minus enzyme, is formulated into convenient one-tube master mix with a simplified protocol, enabling consistency and maximum control of RT-qPCR. It was developed through molecular evolution to improve thermostability, robustness and synthesis rates. Maxima H Minus RT also shows up to a 50x increase in processivity, has no RNase H activity, and demonstrates higher sensitivity.

• Efficiently transcribes large and small amounts of RNA
• Quick and convenient setup eliminates pipeting caused variability
• Reduces risk of cross-contamination between samples
• Consistently higher sensitivity for wide variety of gene targets
• High linearity in cDNA synthesis over a broad dynamic range
• Complete enzyme activity from 42° to 55°C

Specifications

• Kit Type: H Minus cDNA Synthesis Master Mix Kit
• PCR Method: qPCR
• Volume: 200 μL
• Product Type Specs: cDNA Synthesis Master Mix
• Reverse Transcriptase: Maxima H Minus
• Product Line: Maxima&trade
• Optimal Reaction Temperature: 50°C inculbation temperature for RT reactions
• Reaction Format: SuperMix or Master Mix

• Format: Tube
• Sample Type: RNA
• Sufficient For: 500 Reactions
• Concentration: 5X
• Shipping Condition: Dry Ice
• For Use With (Application): Real-Time PCR
• No. of Reactions: 50