Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit

Synthesize long cDNAs up to 20 kb at elevated temperatures (up to 65°C) with this complete system for highly efficient synthesis of first strand cDNA.

Supplier:  Thermo Scientific™ K1652

 View more versions of this product

Catalog No. FERK1652

Add to cart



Maxima H Minus First Strand cDNA Synthesis Kit† is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

The new Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific dsDNase activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.


  • Integrated genomic DNA removal step
  • Increased reaction temperatures -the first strand of cDNA can be synthesized within the 42 to 65°C temperature range
  • High yields of full-length first strand cDNA -with RNA templates up to 20 kb
  • Flexible priming -oligo(dT)18, random hexamer, or gene-specific primers

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.



• Maxima H Minus Enzyme Mix
• Oligo(dT)18 and Random hexamer primers
• 5X RT Buffer
• dNTP Mix
• Nuclease-free water

Store at –20°C.

First-Strand cDNA Synthesis Kit
100 Reactions
Separate components
Reverse Transcription
50°C to 55°C
First-Strand cDNA
30 min.
Up to 20 kb
Reverse Transcription
Maxima H Minus
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)


Product Certifications


Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit