Thermo Scientific™ Maxima H Minus Reverse Transcriptase Promotion

A highly thermostable, high processivity reverse transcriptase lacking RNase H activity

Manufacturer:  Thermo Scientific™ EP0751

Catalog No. FEREP0751PM

Description

• Thermostable - 90% active after incubation at 50°C for 60 minutes in a reaction mixture.
• Active up to 65°C
• High yields of full-length cDNA up to 20 kb
• High sensitivity - reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 µg)
• Efficient - complete cDNA synthesis in 15 to 30 minutes
• Increased resistance to common reaction inhibitors
• Incorporates modified nucleotides

Specifications

• For Use With (Application): First strand cDNA synthesis for RT-PCR and real-time RT-PCR, reverse transcription at elevated temperatures to reduce effects of secondary structure, synthesis of cDNA for cloning and expression, generation of labeled cDNA probes for microarrays
• Storage Requirements: -20°C
• Includes: 5X RT (250mM Tris-HCl buffer (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT)
• Quantity: 2000U