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Thermo Scientific™ Maxima H Minus Reverse Transcriptase Promotion

A highly thermostable, high processivity reverse transcriptase lacking RNase H activity

Manufacturer:  Thermo Scientific™ EP0751

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Catalog No. FEREP0751PM


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Description

Description

  • Thermostable - 90% active after incubation at 50°C for 60 minutes in a reaction mixture.
  • Active up to 65°C
  • High yields of full-length cDNA up to 20 kb
  • High sensitivity - reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 µg)
  • Efficient - complete cDNA synthesis in 15 to 30 minutes
  • Increased resistance to common reaction inhibitors
  • Incorporates modified nucleotides
Specifications

Specifications

First strand cDNA synthesis for RT-PCR and real-time RT-PCR, reverse transcription at elevated temperatures to reduce effects of secondary structure, synthesis of cDNA for cloning and expression, generation of labeled cDNA probes for microarrays
-20°C
5X RT (250mM Tris-HCl buffer (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT)
2000U
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