MBD1 Monoclonal Antibody (100B272.1), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA141025

Catalog No. 01-676-441

Description

Suggested positive control: Hela whole cell extract, HeLa cell lysate.

Methyl-CpG-binding domain protein 1 (MBD1) is a member of a family of mammalian proteins that specifically bind methylated regions of DNA. It is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). MBD1 acts as a transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

Specifications

• Antigen: MBD1
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: Q9UIS9, Q9Z2E2
• Gene Symbols: MBD1
• Immunogen: A synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: ChIP Assay, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: 100B272.1
• Conjugate: Unconjugated
• Gene: MBD1
• Gene Alias: CXXC3; CXXC-type zinc finger protein 3; Mbd1; methyl-CpG binding domain protein 1; methyl-CpG binding domain protein 1 isoform PCM1; methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; PCM1; Protein containing methyl-CpG-binding domain 1; RFT; the regulator of fibroblast growth factor 2 (FGF-2) transcription
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 17190, 4152
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov