MBD2 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA5120803

Catalog No. PIPA5120803

Description

Positive test controls include: mouse heart, mouse kidney, rat heart. The target is usually found in the following locations: Nucleus. Immunogen sequence: DLSSFDFRTG KMMPSKLQKN KQRLRNDPLN QNKGKPDLNT TLPIRQTASI FKQPVTKVTN HPSNKVKSDP QRMNEQPRQL FWEKRLQGLS ASDVTEQIIK TMELPKGLQG VGPGSNDETL LSAVASALHT SSAPITGQVS AAVEKNPAVW LNTSQPLCKA FIVTDEDIRK QEERVQQVRK KLEEALMADI LSRAADTEEM DIEMDSGDEA

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.

Specifications

• Antigen: MBD2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Mbd2
• Gene Alias: demethylase; DKFZp586O0821; DMTase; MBD2; MBD2a; methyl-CpG binding domain protein 2; methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY-CO-41
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17191, 680172
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 1.12 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q9Z2E1
• Gene Symbols: Mbd2
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 202-411 of human MBD2 (NP_003918.1).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG