Invitrogen™ MBL (MBL2) Human ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ EHMBL2

Catalog No. EHMBL2

Description

The Human MBL(MBL2) ELISA quantitates Hu MBL in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MBL. Principle of the method The Human MBL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Mannan-binding lectin (MBL; also called mannose binding lectin or protein) is a multimeric carbohydrate-binding protein produced in the liver and secreted into the blood, where it constitutes an important element in innate immune defense against invading microorganisms. Its normally oligomerized forms are associated with specific serine proproteases (the MASPs) which are activated when MBL binds to microbial carbohydrate surfaces and in turn activate complement via the MBL or lectin pathway. The determination of MBL concentrations in serum may be useful for the elucidation of suspected immune defects and as a prognostic indicator alerting to the need for heightened therapeutic or prophylactic measures in immunosuppressed patients, including patients receiving cancer chemotherapy, and patients with cystic fibrosis, SLE or rheumatoid arthritis.

Specifications

• Accession Number: P11226
• Assay Sensitivity: 0.03 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 4153
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Microbiology
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 0.034-25 ng/mL
• Calibration Range: 0.03ng/mL to 25ng/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Specificity: This ELISA antibody pair detects human MBL. Other species not determined yet.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: COLEC1,HSMBPC,MBLD,MBP,MBP-C,MBP1,MBPD,MBL2
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL
• Synonym: RP11-94H3.1, COLEC1, HSMBPC, MBLD, MBP, MBP-C, MBP1, MBPD, MBL2
• Test Time: 1 hr. 20 min.