Invitrogen™ MCM4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA578055

Catalog No. PIPA578055

Description

Positive Control: Jurkat, Raji, NCI-H929, 293T, A431, HeLa, HepG2, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12 Predicted Reactivity: Rat (99%), Zebrafish (87%), Xenopus laevis (92%), Rhesus Monkey (99%), Bovine (97%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Specifications

• Antigen: MCM4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Mcm4
• Gene Alias: 19G; AI325074; AU045576; cb1025; Cdc21; CDC21 homolog; CDC54; DNA replication licensing factor MCM4; fc12c09; fj85g09; hCdc21; hm:zeh1616; homolog of S. pombe cell devision cycle 21; mcdc21; MCM4; MCM4 minichromosome maintenance deficient 4; MCM4 minichromosome maintenance deficient 4, mitotin; mcm4 protein; Mcmd4; mini chromosome maintenance deficient 4 homolog; minichromosome maintenance complex component 4; minichromosome maintenance deficient 4; minichromosome maintenance deficient 4 homolog; minichromosome maintenance deficient 4 homolog (S. cerevisiae); mKIAA4003; NKCD; NKGCD; P1-CDC21; Unknown (protein for MGC:140484); wu:fc12c09; wu:fj85g09; zeh1616
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17217, 4173
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 2.49 mg/mL
• Formulation: PBS with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P33991, P49717
• Gene Symbols: Mcm4
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MCM4. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov