MCPT-1 (mMCP-1) Monoclonal Antibody (RF6.1), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 14550382

Catalog No. 50-245-560

Description

Description: The monoclonal antibody RF6 recognized MCPT-1 (Mast Cell Protease-1) which is a β-chymase, a type of serine protease stored and secreted in a tissue-specific manner by mucosal mast cells. MCPT-1 is a chymotryptic protease, referring to its ability to cleave proteins and peptides after an aromatic amino acid. It shares 74% sequence homology with its rat counterpart, rat Mast Cell Protease-II (rMCP-II) and has no direct human counterpart. MCPT-1 is the only chymase expressed by intestinal mucosal mast cells, which are found in the intestinal epithelium. Although it is expressed constitutively and is detectable in the sera of normal mice, parasites in the gut cause systemic levels to increase dramatically within two days and peak at two weeks following infection. It plays an important role in host defense against intestinal parasites, as mice deficient in MCPT-1 display delayed response upon infection. Elevated MCPT-1 levels are also observed during intestinal allergic hypersensitivity reactions. The mechanism of its action is not fully understood, although it is believed to increase intestinal permeability similarly to rMCP-II, possibly by cleaving the proteins at tight junctions between cells. Applications Reported: This RF6 antibody has been reported for use in immunocytochemistry and ELISA. Applications Tested: This RF6 antibody has been tested by ELISA.

MCPT-1 is the only chymase expressed by intestinal mucosal mast cells, which are found in the intestinal epithelium. Although it is expressed constitutively and is detectable in the sera of normal mice, parasites in the gut cause systemic levels to increase dramatically within two days and peak at two weeks following infection. It plays an important role in host defense against intestinal parasites, as mice deficient in MCPT-1 display delayed response upon infection. Elevated MCPT-1 levels are also observed during intestinal allergic hypersensitivity reactions. The mechanism of its action is not fully understood, although it is believed to increase intestinal permeability similarly to rMCPT-II, possibly by cleaving the proteins at tight junctions between cells.

Specifications

• Antigen: MCPT-1 (mMCP-1)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P11034
• Gene Symbols: Mcpt1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17224
• Content And Storage: 4° C
• Form: Liquid

• Applications: ELISA, Immunocytochemistry, Immunohistochemistry
• Clone: RF6.1
• Conjugate: Unconjugated
• Gene: Mcpt1
• Gene Alias: AV080368; mast cell protease 1; Mcp-1; Mcpt1; mMCP-1
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1

For Research Use Only.