Description: The 9B4 antibody reacts with human MD-2, an approximately 30 kDa molecule, which physically associates with the extracellular domain of TLR4. MD-2 is essential for correct intracellular distribution and LPS-recognition of TLR4 since in MD-2(-/-) embryonic fibroblasts, TLR4 was not able to reach the plasma membrane and predomitly resided in the Golgi apparatus, whereas TLR4 was distributed at the leading edge surface of cells in wild-type embryonic fibroblasts. MD-2(-/-) mice do not respond to LPS, do survive endotoxic shock but are susceptible to Salmonella typhimurium infection. Applications Reported: The 9B4 antibody has been reported for use in immunoprecipitation. Applications Tested: This 9B4 antibody has been tested by immunoprecipitation of recombit MD-2 from cell supernatant. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
|PBS with 0.09% sodium azide; pH 7.2|
For Research Use Only.
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