Invitrogen™ MegaX DH10B T1R Electrocomp™ Cells
Highest-efficiency electrocompetent cells available
Manufacturer: Invitrogen™ C640003
Improved production processes guarantee a three-fold greater number of colonies per transformation (>3 x 1010 cfu/μg of pUC DNA), making the MegaX DH10B™ T1R cells ideal for highly demanding cloning and library construction. Same genotype as the widely used DH10B™ T1R strain, including tonA, which prevents infection by T1 and T5 lytic bacteriophages, and safeguards valuable clones and libraries.
- Provide highly efficient transformation of methylated DNA, since they lack E. coli K12 restriction systems
- ψ80lacZδM15 marker provides α-complementation of β-galactosidase gene, allowing blue/white screening on agar plates containing X-gal or Bluo-gal
- Bacterial/Yeast Strain: DH10B™
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Shipping Condition: Dry Ice
|Lacks F' Episome|
|Not Suitable for preparing unmethylated DNA|
|Not Suitable for cloning unstable DNA|
• MegaX DH10B T1R Electrocomp C: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• Recovery Medium: 3 bottles, 10 ml each
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store Recovery Medium at 4°C .
|Not High-throughput Compatible (Manual)|
|May be used for plasmids > 100 kb|
|Not for ccdB vector propagation|
|High Efficiency (> 10^9 cfu⁄µg)|
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For Research Use Only. Not for use in diagnostic procedures.