Melan-A/MART-1 Antibody (M2-9E3), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 2 publications

Catalog Number	Mfr. No.	Quantity
NBP21519801	Novus Biologicals NBP2151980.1MG	0.1 mg

Description

Melan-A/MART-1 Monoclonal specifically detects Melan-A/MART-1 in Human, Mouse, Rat samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Melan-A/MART-1
• Concentration: 0.2mg/mL
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Gene Accession No.: Q16655
• Gene ID (Entrez): 2315
• Immunogen: Recombinant human Melan-A protein was used as immunogen to generate the Melan-A antibody.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: M2-9E3
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, SDS-Page
• Conjugate: Unconjugated
• Host Species: Mouse
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: Antigen LB39-AA, Antigen SK29-AA, Mart 1 Melan A, MART1MART-1, melan-A, melanoma antigen recognized by T-cells 1, Protein Melan-A
• Gene Symbols: MLANA
• Isotype: IgG2b κ
• Purification Method: Protein G purified
• Test Specificity: This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

For Research Use Only