Expression profiling using gene arrays has become the most widely used method for assessing gene expression on a genome-wide scale. The primary limitation of this technology is the requirement for large amounts of RNA for array hybridization. This hurdle is usually overcome by using T7 RNA Polymerase–driven transcription of cDNA (obtained from total RNA samples) to amplify sufficient amounts of cRNA. This amplification method is the basis of the MessageAmp™ II-Biotin Enhanced aRNA Amplification Kit.
Improvements for Biotin Labeling
The IVT reaction step of the MessageAmp II-Biotin Enhanced Kit procedure has been optimized for effective biotin labeling. An NTP mix containing biotinylated UTP has been added to the kit for ease of use. Other optimizations have increased sensitivity and increased yields so that just 50ng of input RNA is sufficient to produce enough aRNA for array analysis after just a single round of amplification. These kits do not include enough reagents for two rounds of amplification. If your experiments require two rounds of amplification, you will need to purchase the MessageAmp II aRNA Amplification Kit in addition to the MessageAmp II-Biotin Kit.
The MessageAmp II aRNA Amplification Kit (Cat. No. AM1751) is available should additional reagents be needed for two rounds of aRNA amplification.
- An included NTP mix contains biotinylated UTP
- Includes sufficient reagents for 100 single-round aRNA amplifications
- Detect more genes due to increased percent present calls
- Obtain sufficient amounts of amplified RNA from a single round of amplification with only 50ng of input RNA
- Biotinylated nucleotide is included in the optimized NTP mix, reducing setup time and maximizing labeling
Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, RNA Amplification
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