The Mouse MFG-E8 ELISA quantitates Ms MFG-E8 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MFG-E8. Principle of the method The Mouse MFG-E8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.Human milk fat globule membrane protein (HMFG1) is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG1 is considered as a differentiation marker. It is useful as specific breast epithelial marker in normal and neoplastic mammary development.
|2°C to 8°C|
|Plasma, 20 μL; Serum, 20 μL; Supernatant, 50 μL|
|1 hr. 20 min.|
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