MGMT (O-methylguanine-DNA methyltransferase) Ab-1 Mouse Monoclonal Antibody, Epredia™

Epredia™ MGMT (O-methylguanine-DNA methyltransferase) Ab-1, Mouse Monoclonal Antibody provides accurate, reproducible results.

Supplier:  Epredia MS470P1

Catalog No. MS470P1

Description

Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross-links at the O-alkylguanine position in DNA. MGMT transfers alkyl adducts from the O-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely be drug-resistant.

Host Species: Mouse

Clone: MT 3.1

Isotype: IgG1

Species Reactivity: Human. Also reacts weakly with Mouse, Rat and Hamster. Others not known.

Epitope: aa 8-221

Immunogen: Purified recombinant human MGMT protein

Molecular Weight: 25kDa

Positive Control: Raji cells, Colon CA

Cellular Localization: Nuclear and cytoplasmic

Recommended for:

• Immunohistochemistry (Formalin/paraffin)
• Western Blotting
• Flow Cytometry

Note:

USA: RUO; Int'l: RUO

Specifications

• Antigen: MGMT (O-methylguanine-DNA methyltransferase) Ab-1
• Classification: Monoclonal
• Concentration: 200μg/mL
• Host Species: Mouse
• Quantity: 500 μL
• Research Discipline: Enzyme
• Target Species: Hamster, Human, Mouse, Rat
• Isotype: IgG1

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: MT 3.1
• Conjugate: Unconjugated
• Immunogen: Purified recombinant human MGMT protein
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Form: Concentrated