MHC Class I (H-2Kd/H-2Dd) Mouse anti-Mouse, PerCP-eFluor 710, Clone: 34-1-2S, eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 46599880
Description: The 34-1-2S monoclonal antibody reacts with the mouse MHC class I, H-2Kd and H-2Dd. This cytotoxic antibody also cross-reacts with Kb, s, r, q, p. Applications Reported: This 34-1-2S antibody has been reported for use in flow cytometric analysis. Applications Tested: This 34-1-2S antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.
|MHC Class I (H-2Kd/H-2Dd)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|H-2 class I histocompatibility antigen, K-D alpha chain, H2K1, H2KD, H2DD|
|4° C, store in dark, DO NOT FREEZE!|
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