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MHC Class II I-Ab Monoclonal Antibody (AF6-120.1), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Supplier: Invitrogen 46532082
Description
Description: This AF6-120.1.2 monoclonal antibody reacts with the mouse MHC Class II I-Ab alloantigen of H-2b bearing mouse strains, including C57Bl/6 and 129. This cell surface molecule is involved in antigen presentation to T cells expressing CD3/TCR and CD4. The AF6-120.1 antibody crossreacts with H-2k and H-2u haplotypes. However, reactivity to other haplotypes (i.e., d, f, g7, p, q, r, and s) has not been observed. Applications Reported: This AF6-120.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AF6-120.1 antibody has been tested by flow cytometric analysis of C57Bl/6 splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
MHC Class II I-Ab Monoclonal antibody specifically detects MHC Class II I-Ab in Mouse samples. It is validated for Flow Cytometry.Specifications
MHC Class II I-Ab | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P01921, P06342, P06343, P06344, P06345, P06346, P14483 | |
H2-Ab1 | |
Affinity chromatography | |
RUO | |
14961 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
AF6-120.1 | |
PerCP-eFluor 710 | |
H2-Ab1 | |
Abeta; AI845868; CELIAC1; DADB-249P12.2; H-2 class II histocompatibility antigen, A beta chain; H-2Ab; H2-Ab; H2-Ab1; H2Eb; H-2Eb; H2-iabeta; histocompatibility 2, class II antigen A, beta 1; HLA-DQB; Ia2; Ia-2; Ia4; Ia-4; IAb; I-Abeta; IDDM1; major histocompatibility complex class II beta chain; MGC163794; MGC163796; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1; Rmcs1 | |
Mouse | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
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For Research Use Only.