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MHC Class II Mouse anti-Rat, APC, Clone: HIS19, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 17092082

Catalog No. 50-150-38


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Description

Description

Description: The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except the RT1n (including BN and MAXX strains). The rat MHC class II (I-A equivalent), RT1B, is expressed by antigen presenting cells including B cells, dendritic cells and macrophages. Applications Reported: This HIS19 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIS19 antibody has been tested by flow cytometric analysis of rat spleen or lymph node cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Specifications

Specifications

MHC Class II
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Major histocompatibility complex class II; MHC 2; MHC Class II; MHC II; MHCCII
IgG1
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
Rat
Flow Cytometry
HIS19
APC
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
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