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MHC Class II Monoclonal Antibody (YKIX334.2), APC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Supplier: Invitrogen 17590942
Description
Description: This YKIX334.2 monoclonal antibody reacts with canine major histocompatibility complex (MHC) Class II. The MHC plays a significant role in immunity by presenting self and non-self antigens to T cells. In dogs, MHC Class II is expressed on all T and B cells, macrophages, fibroblasts, and kidney interstitial cells. This monoclonal antibody has been reported to inhibit mixed lymphocyte reactions and T cell activation induced by MHC Class II antigen presentation in vitro. Also, YKIX334.2 recognition of MHC Class II is not allele-specific. Applications Reported: This YKIX334.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This YKIX334.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.
MHC Class II Monoclonal antibody specifically detects MHC Class II in Canine samples. It is validated for Flow Cytometry.Specifications
MHC Class II | |
Monoclonal | |
5 μL/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
Rat | |
100 Tests | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
YKIX334.2 | |
APC | |
beta 1 domain; B-L; B-L beta chain; B-L beta minor; B-LB; BLB major; BLB1; BLB2; B-LB2; B-LB21; B-LB21 major; BLB3; Bl-Beta II protein; B-LBII; class II histocompatibility antigen, B-L beta chain; class II histocompatibility antigen, M alpha chain; D6S222E; DMA; FECA-DRB; GSP-BLB1; GSP-BLB2; HLA class II histocompatibility antigen, DM alpha chain; HLADM; HLA-DMA; major histocompatibility class II antigen B-L beta; major histocompatibility complex class II B; Major histocompatibility complex class II beta chain BLB1; Major histocompatibility complex class II beta chain BLB1, (similar to HLA class II, D beta chain); major histocompatibility complex class II beta chain BLB1, (similar to HLA class II, D beta chain); Major histocompatibility complex class II beta chain BLB2, (similar to HLA class II, D beta chain); Major histocompatibility complex class II beta chain BLB3, (similar to HLA class II, D beta chain); major histocompatibility complex, class II, DM alpha; MHC 2; MHC Class II; MHC | |
Affinity chromatography | |
RUO | |
Canine | |
Antibody | |
IgG2a κ |
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For Research Use Only.