MHC Class II Rat anti-Canine, FITC, Clone: YKIX334.2, eBioscience
Rat Monoclonal Antibody
Manufacturer: Invitrogen 11590942
Description: This YKIX334.2 monoclonal antibody reacts with canine major histocompatibility complex (MHC) Class II. The MHC plays a significant role in immunity by presenting self and non-self antigens to T cells. In dogs, MHC Class II is expressed on all T and B cells, macrophages, fibroblasts, and kidney interstitial cells. This monoclonal antibody has been reported to inhibit mixed lymphocyte reactions and T cell activation induced by MHC Class II antigen presentation in vitro. Also, YKIX334.2 recognition of MHC Class II is not allele-specific. Applications Reported: This YKIX334.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This YKIX334.2 antibody has been pre-titrated and tested by flow cytometric analysis of canine peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
|MHC Class II|
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Major histocompatibility complex class II; MHC 2; MHC Class II; MHC II; MHCCII|
|4° C, store in dark, DO NOT FREEZE!|
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