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MHC I Free Chain Mouse anti-Human, Clone: A4, eBioscience™ Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  LIFE TECHNOLOGIES 14995837


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Catalog No. 14-995-837

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The A4 monoclonal antibody reacts with the class I human leukocyte antigen (HLA) complex in the absence of beta2 microglobulin. There are three class I alpha-chain genes in humans, called HLA-A, -B and -C. Normally, HLA class I proteins are associated non-covalently with beta2 microglobulin (beta2M). The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen specific CD8+ cytotoxic T cells. Class I HLA is expressed by the majority of nucleated cells. Applications Reported: This A4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of paraffin embedded tissue sections. Applications Tested: This A4 antibody has been tested by flow cytometric analysis of 3-day PHA-activated normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

MHC I Free Chain Monoclonal antibody specifically detects MHC I Free Chain in Human samples. It is validated for Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation


MHC I Free Chain
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
2 mg
4° C
Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation
MHC Free
Affinity chromatography
IgG1 κ
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