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MHC I Free Chain Mouse anti-Human, Clone: A4, eBioscience™ Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: LIFE TECHNOLOGIES 14995882
Description
The A4 monoclonal antibody reacts with the class I human leukocyte antigen (HLA) complex in the absence of beta2 microglobulin. There are three class I alpha-chain genes in humans, called HLA-A, -B and -C. Normally, HLA class I proteins are associated non-covalently with beta2 microglobulin (beta2M). The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen specific CD8+ cytotoxic T cells. Class I HLA is expressed by the majority of nucleated cells. Applications Reported: This A4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of paraffin embedded tissue sections. Applications Tested: This A4 antibody has been tested by flow cytometric analysis of 3-day PHA-activated normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
MHC I Free Chain Monoclonal antibody specifically detects MHC I Free Chain in Human samples. It is validated for Flow Cytometry, Immunohistochemistry (Paraffin), ImmunoprecipitationSpecifications
| MHC I Free Chain | |
| Monoclonal | |
| 0.5 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| Mouse | |
| 100 μg | |
| Primary | |
| Antibody | |
| IgG1 κ |
| Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation | |
| A4 | |
| Unconjugated | |
| MHC Free | |
| Affinity chromatography | |
| RUO | |
| Human | |
| Liquid |
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