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MHC I Free Chain Monoclonal Antibody (A4), eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
$231.00 - $1372.00
Specifications
Antigen | MHC I Free Chain |
---|---|
Clone | A4 |
Concentration | 0.5 mg/mL |
Applications | Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation |
Classification | Monoclonal |
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
14-995-882
|
LIFE TECHNOLOGIES
14995882 |
100 μg |
Each for $231.00
|
|
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14-995-837
|
LIFE TECHNOLOGIES
14995837 |
2 mg |
Each for $1,372.00
|
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Description
Description: The A4 monoclonal antibody reacts with the class I human leukocyte antigen (HLA) complex in the absence of beta2 microglobulin. There are three class I alpha-chain genes in humans, called HLA-A, -B and -C. Normally, HLA class I proteins are associated non-covalently with beta2 microglobulin (beta2M). The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen specific CD8+ cytotoxic T cells. Class I HLA is expressed by the majority of nucleated cells. Applications Reported: This A4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of paraffin embedded tissue sections. Applications Tested: This A4 antibody has been tested by flow cytometric analysis of 3-day PHA-activated normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
There are three class I alpha-chain genes in humans, called HLA-A, -B and -C. Normally, HLA class I proteins are associated non-covalently with B2 microglobulin (B2M). The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen specific CD8+ cytotoxic T cells. Class I HLA is expressed by the majority of nucleated cells.Specifications
MHC I Free Chain | |
0.5 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
PBS with 0.09% sodium azide; pH 7.2 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
A4 | |
Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation | |
Unconjugated | |
Mouse | |
Human | |
MHC Free | |
Primary | |
4° C |
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