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Invitrogen™ MIF Human ELISA Kit Non-distribution product as customer accommodation.

Sandwich ELISA Kit

$494.00

Specifications

Storage Requirements 2°C to 8°C
Assay Sensitivity 6 pg/mL
Assay Range 8.23-6000 pg/mL
Test Time 1 hr. 20 min.
Sample Volume Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
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Catalog Number Mfr. No. Quantity Price Quantity & Availability  
EHMIF Non-distribution product as customer accommodation.
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Invitrogen™
EHMIF
96 Tests
Pack for $494.00
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Description

Description

The Human Macrophage Inflammatory Factor (Hu MIF) ELISA quantitates Hu MIF in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MIF. Principle of the method The Human MIF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MIF (Macrophage Migration Inhibitory Factor) is a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through suppression of the anti-inflammatory effects of glucocorticoids. MIF may also play a role in integrin signaling pathways, because it has been found to form a complex in the cytosol near the peripheral plasma membrane with the JAB1 protein.
Specifications

Specifications

2°C to 8°C
8.23-6000 pg/mL
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
<10%
6 pg/mL
1 hr. 20 min.
<12%
SDS
Product Certifications
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