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Invitrogen™ MIP-1 alpha (CCL3) Mouse ELISA Kit Non-distribution product as customer accommodation.

Sandwich ELISA Kit

$494.00

Specifications

Storage Requirements 2°C to 8°C
Assay Sensitivity 8 pg/mL
Assay Range 4.1-1000 pg/mL
Test Time 1 hr. 20 min.
Sample Volume Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
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Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
EMCCL3 Non-distribution product as customer accommodation.
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Invitrogen™
EMCCL3
96 Tests
Pack for $494.00
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Description

Description

The Mouse MIP-1alpha (CCL3) ELISA quantitates Ms MIP-1α in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MIP-1α. Principle of the method The Mouse MIP-1α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CCL3 (MIP1a, Macrophage Inflammatory Protein-1 alpha, MIP-1 alpha) has a molecular weight of approximately 8 kDa, belongs to the family of chemotactic cytokines, and is involved in chemotactic and proinflammatory effects, and homeostasis. CCL3 is produced by lymphocytes, macrophages and dendritic cells, and is involved in inflammatory response of blood monocytes and tissue macrophages. Also, CCL3 can exist as a naturally occurring heterodimer with MIP-1beta and has been shown to have antiviral activity against HSV-1. CCL3 protein can bind with high affinity to the CCR1 and CCR5 receptors.
Specifications

Specifications

2°C to 8°C
4.1-1000 pg/mL
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
<10%
8 pg/mL
1 hr. 20 min.
<12%
SDS
Product Certifications
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