BioVision Inc Mitochondrial Apoptosis Detection Fluorometric Kit, MitoCapture: Simple, Fluorescent-based method to distinguish between Healthy & Apoptotic Cells by detecting change in the mitochondrial transmembrane potential. Kit Size: 25 & 100 Assays.
Manufacturer: BioVision Inc K25025
Mitochondrial Apoptosis Detection Fluorometric Kit: is one of the earliest intracellular events that occur following induction of apoptosis. The MitoCapture¿ Apoptosis Detection Kit provides a fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial potential. The kit utilizes MitoCapture¿, a cationic dye that fluoresces differently in healthy vs apoptotic cells. In healthy cells, MitoCapture accumulates and aggregates in the mitocondria, giving off a bright red fluorescence. In apoptotic cells, MitoCapture cannot aggregate in the mitochondria due to the altered mitochondrial transmembrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530+ 30 nm) and (optional) PI channel for red aggregates (Em = 488/590+ 42 nm).
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