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GE Healthcare MMP-1 Human Biotrak ELISA System Available on GSA/VA Contract for Federal Government customers only.

Measure total human MMP-1 levels in one day. GE Healthcare MMP-1 Human Biotrak ELISA System is a ready-to-use kit featuring a coated 96-well microplate and all required buffers and standards. Biotrak ELISAs measure MMP protein levels in human serum, plasma and cell culture samples.

Manufacturer:  GE Healthcare RPN2610

Catalog No. 45-200-587


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Description

Description

The MMP-1 Human Biotrak ELISA system has been specifically designed for research purposes. MMP-1 may be measured in the range 6.25-100ng/ml. The assay is based on a two site ELISA ‘sandwich’ format.
  • Measures total MMP-1
  • Specific for human MMP-1
  • Precise and accurate measurement
  • Non-isotopic protocol
  • Ready to use substrate
  • Same day protocol
Measurement protocol
  • Standards and samples are incubated in microplate wells pre-coated with anti-MMP-1 antibody
  • Any MMP-1 present will be bound to the wells, with other components of the sample being removed by washing and aspiration
  • A second polyclonal antibody to MMP-1 is then bound to the MMP-1; any excess is removed by washing and aspiration
  • The second antibody bound to the wells is detected using donkey anti-rabbit horseradish peroxidase
  • The amount of peroxidase bound to each well is determined by the addition of TMB ‘ready-to-use’ substrate
  • The reaction is stopped by addition of an acid solution and the resultant color read at 450nm in a microplate spectrophotometer
The concentration of MMP-1 in a sample is determined by interpolation from a standard curve. Each pack contains reagent for 96 determinations. This allows the construction of a standard curve plus the measurement of 42 samples in duplicate.

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Specifications

Specifications

6.25ng/mL to 100ng/mL
MMP-1, Human, Biotrak Assay.
Pipettes or pipetting equipment with disposable polypropylene tips (100 μl, 500 μl, 1 mL and 5 mL); Disposable polypropylene test tubes; Glass measuring cylinders (100 mL, and 1 l); Distilled or deionized water; Spectrophotometer plate reader capable of m
PP (Polypropylene)
1.7 ng/ml
-15 - -30°C
0.079
Suitable for use with serum, plasma (EDTA, heparin, citrate) and cell culture supernatant samples Biotrak ELISAs measure MMP protein levels in human serum, plasma, and cell culture samples
Microplate: 12 x 8 well strips coated with mouse anti-MMP-1; Assay buffer 1: 10mL of phosphate buffer concentrate; Assay buffer 2: 10mL of phosphate buffer concentrate; Standard: 1mL human MMP-1 (recombinant); Antibody: lyophilised rabbit anti-MMP-1; Peroxidase conjugate: lyophilised donkey anti-rabbit horseradish peroxidase; Wash buffer: 12.5mL phosphate buffer concentrate; TMB substrate: 3,3’,5,5’-Tetramethylbenzidine (TMB)/hydrogen peroxide
96 wells
The assay recognises total human MMP-1, ie. free MMP-1 and that complexed with inhibitors such as TIMP-1. It does not recognise MMP-1 bound by the non-specific protease inhibitor α2-macroglobulin. There is no cross-reactivity with α2-macroglobulin alone.
5.5-h
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