Invitrogen™ ActinRed™ 555 ReadyProbes™ Reagent (Rhodamine phalloidin)

Selective, high-affinity F-actin probe conjugated to red-orange fluorescent dye tetramethylrhodamine (TRITC)

Supplier:  Invitrogen™ R37112

Catalog No. R37112

Includes: 2 x 2.5mL dropper bottles

Description

• Formulated as room temperature-stable solution and comes in convenient dropper bottle: tip and drip2 drops/mL to stain cells
• High-affinity staining of F-actin with superior specificity compared to antibody methods
• Outstanding fluorescence performance (Ex/Em: 540/565nm)
• Ready-to-use liquid formulation: no need to dilute, weigh, or pipet
• Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin
• Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low nonspecific binding
• Phalloidin binds F-actin with high selectivity, while TRITC provides red-orange fluorescence of unparalleled brightness and photostability
• Demonstrating very little nonspecific staining, allows high-contrast discrimination of actin staining
• Visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations
• Fully compatible with other fluorescent stains used in fixed-cell analyses, including antibodies, fixed-cell dyes, fluorescent proteins, and Qdot probe
• Validated and robust reagent for studying cytoskeleton in the context of stem cell differentiation, cancer biology, as marker in RNAi library screens, or target in drug discovery
• May be added directly to fixed cells in full media or buffer solutions
• Optimization may be needed for some cell types, conditions, and applications: In such cases, simply add more or fewer drops until the optimal staining intensity is obtained
• Detected through standard TRITC (Rhodamine/DiI/Cy3/Alexa Fluor 546) filters

Cell Analysis, Cell Structure, Cytoskeleton

Specifications

• Color: Red-Orange
• Excitation Wavelength Range: 540⁄565
• For Use With (Equipment): Confocal Microscope, EVOS™, Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, Floid™ Cell Imaging System
• Detection Method: Fluorescent
• Form: Liquid
• Technique: Fluorescence Intensity
• SubCellular Localization: Cytoskeleton

• Content And Storage: 2 × 2.5 mL dropper bottles
  
  Store at ≤ 25°C.
  
  
• Dye Type: TRITC (Tetramethylrhodamine Isothiocyanate)
• Quantity: 2 dropper bottle(s)
• Emission: Visible
• Product Line: ActinRed™, ReadyProbes™
• Product Type: Phalloidin

For Research Use Only. Not for use in diagnostic procedures.