GFP Rabbit anti-Tag, HRP, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen A10260
We offer the anti-GFP antibody HRP as purified rabbit IgG fraction and conjugated to horseradish peroxidase (HRP). At the time of preparation, the products are certified to be free of unconjugated dyes and are tested in a cytological experiment to ensure low nonspecific staining. This anti-GFP antibody HRP conjugate is suited for detection of native GFP, GFP variants, and most GFP-fusion proteins by western blot analysis and immunocytochemistry. For initial experiments, we recommend trying dilutions ranging from 1:200 to 1:2,000 for immunocytochemical applications and western blot analysis. It is a good practice to centrifuge the protein conjugate solutions briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step eliminates any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. To prepare 0.2 mg/mL stock solutions, reconstitute the lyophilized antibody in 0.5 mL of phosphate-buffered saline (PBS), pH 7.4. Store solution at 2-8°C with the addition of thimerosal to a final concentration of 0.02%. For prolonged storage after reconstitution, add glycerol to a final concentration of 50% (v/v), aliquot, and store at -20°C. When stored properly, the solution is stable for approximately three months.Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP), from the jellyfish Aequorea victoria, has quickly become a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression, and a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
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Shipping Condition: Wet Ice
|PBS with no preservative; pH 7.4|
|GFP2; Green Fluoresecent Protein; GFP; Turbo GFP; eGFP; GFP tag|
|The GFP was isolated directly from the jellyfish Aequorea victoria.|
|Immunohistochemistry, Western Blot|
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For Research Use Only. Not for use in diagnostic procedures.