Invitrogen™ Qtracker™ 705 Cell Labeling Kit

Designed for loading cells grown in culture with highly fluorescent Qdot nanocrystals

Supplier:  Invitrogen™ Q25061MP

Catalog No. Q25061MP

Includes: 100μL Qtracker nanocrystals, Component A (2μM in 50mm borate buffer, pH 8.3) and 100μL Qtracker carrier, Component B (phosphate buffered saline, pH 7.2).

Description

• Use a proprietary targeting peptide to deliver near-infrared-fluorescent Qdot 705 nanocrystals into the cytoplasm of live cells
• Once inside the cells, Qtracker labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in a population
• Provide intense, stable fluorescence that can be traced through several generations
• Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays
• Cytoplasmic delivery by this mechanism is not mediated by a specific enzyme; therefore, no cell-type specificity has been observed. Delivery is typically accomplished in less than 1 hour
• Nanocrystals delivered are compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity
• Furthermore, autofluorescence commonly observed in cells or tissues can be avoided
• Observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes

• Fluorescence from the can be seen up to a week after delivery in some cell lines
• Can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems
• Cytotoxicity of the materials has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells: Labeling appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility
• Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics

Cell Analysis, Cell Tracing and Tracking, General Cell Tracing, Neuronal Tracing

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Contains 100 μl Qtracker™ nanocrystals, Component A (2 μM in 50 mM borate buffer, pH 8.3) and 100 μl Qtracker™ carrier,Component B (phosphate buffered saline, pH 7.2). Store at 2–6°C. Do not freeze. Stable for at least 6 months.
• Reagent Type: Cell Labeling Reagents
• Product Type: Cell Labeling

• Product Line: QTRACKER™, Qdot™
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.