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Invitrogen™ Thiol and Sulfide Quantitation Kit

Ultrasensitive colorimetric assay for quantifying both protein and nonprotein thiols

Manufacturer:  Invitrogen™ T6060


Catalog No. T6060

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In this assay, thiols or inorganic sulfides reduce a disulfide-inhibited derivative of papain, stoichiometrically releasing the active enzyme. The activity of the enzyme is then measured using the chromogenic papain substrate N-benzoyl-L-arginine, p-nitroanilide (L-BAPNA). The enzymatic amplification step in this kit enables researchers to detect as little as 0.2μM thiol - a sensitivity that is about 100-fold greater than that achieved using Ellman's reagent.

Anion Detection, Biochemical Toxicology Assays, Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Drug Discovery and Development, Ionic Homeostasis and Signaling, Target-Based ADME/Tox Assays

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Thiol and Sulfide Quantitation Kit


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For Research Use Only. Not for use in diagnostic procedures.