Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24496200	Novus Biologicals NBP2449620.02MG	0.02 mg
NBP24496201	Novus Biologicals NBP2449620.1MG	0.1 mg
NBP24496202	Novus Biologicals NBP2449620.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence

Alkaline Phosphatase, Tissue Non-Specific Monoclonal specifically detects Alkaline Phosphatase, Tissue Non-Specific in Human, Bovine samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunofluorescence.

Specifications

• Antigen: Alkaline Phosphatase, Tissue Non-Specific
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Frozen), SDS-Page, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Primate
• Gene Accession No.: P05186, P05187
• Gene ID (Entrez): 249
• Immunogen: Bovine intestinal alkaline phosphatase
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 55 kDa

• Clone: V17.1
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935
• Gene Symbols: ALPL
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

For Research Use Only