Mouse anti-Bcl-2, Clone: 100/D5+124, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2345150.2 MG

Catalog No. NBP23451502

Description

Bcl-2 Monoclonal antibody specifically detects Bcl-2 in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

• Antigen: Bcl-2
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
• Gene Accession No.: P10415
• Gene Symbols: BCL2
• Immunogen: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
• Target Species: Human, Mouse (Negative), Rat (Negative)
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Clone: 100/D5+124
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
• Host Species: Mouse
• Molecular Weight of Antigen: 25 kDa
• Quantity: 0.2 mg
• Research Discipline: Apoptosis, Autophagy, Cancer, Cellular Markers, Phospho Specific, Tumor Suppressors
• Gene ID (Entrez): 596
• Reconstitution: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG

For Research Use Only