Mouse anti-c-Myb, Clone: MYB286, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2346900.2 MG

Catalog No. NBP23469002

Description

c-Myb Monoclonal antibody specifically detects c-Myb in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, CyTOF-ready.

Specifications

• Antigen: c-Myb
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, CyTOF-ready
• Gene Accession No.: P10242
• Gene Symbols: MYB
• Immunogen: A synthetic peptide, corresponding to aa 119-135 (RRKVEQEGYPQESSKAG) of c-myb oncoprotein, coupled to KLH
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: The highly leukemogenic avian retrovirus E26 contains two oncogenes, v-Myb and v-Ets, which are expressed together as a fusion protein. The cellular homolog of v-Myb, designated c-Myb, encodes a transcription factor. Deletion or disruption of a negative regulatory domain mapping within the carboxy-terminal domain of c-Myb results in enhanced transactivating capacity and in parallel, leads to activation of its ability to transform hemopoietic cells. c-Myb is expressed preferentially, but not exclusively, in immature hemopoietic cells and its expression decreases as cells differentiate.
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, CyTOF
• Clone: MYB286
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: c-myb, Cmyb, c-myb protein (140 AA), c-myb_CDS, c-myb10A_CDS, c-myb13A_CDS, c-myb14A_CDS, c-myb8B_CDS, efg, Proto-oncogene c-Myb, transcriptional activator Myb, v-myb avian myeloblastosis viral oncogene homolog, v-myb myeloblastosis viral oncogene homolog (avian)
• Host Species: Mouse
• Molecular Weight of Antigen: 75 kDa
• Quantity: 0.2 mg
• Research Discipline: Cancer, Phospho Specific
• Gene ID (Entrez): 4602
• Reconstitution: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only