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Mouse anti-Caldesmon/CALD1, Clone: CALD1/820, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2444230.2MG

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Catalog No. NBP24442302

This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Specifications

Specifications

Caldesmon/CALD1
CALD1/820
Unconjugated
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Purified
CALD1
Recombinant full-length human CALD1 protein
150 kDa
0.2 mg
Cytoskeleton Markers
Primary
800
Human, Rat (Negative)
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
0.2mg/mL
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 1 - 2 ug/ml
Q05682
CAD, caldesmon, caldesmon 1, CDMH-CAD, HCAD, LCAD, L-CAD, MGC21352, NAG22
Mouse
IgG1 Kappa
Protein A or G purified
RUO
Store at 4C.
Monoclonal
Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
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