Calponin 1 Antibody (CNN1/832 + CALP), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24448200	Novus Biologicals NBP2444820.02MG	0.02 mg
NBP24448201	Novus Biologicals NBP2444820.1MG	0.1 mg
NBP24448202	Novus Biologicals NBP2444820.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Calponin 1 Monoclonal specifically detects Calponin 1 in Human, Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Calponin 1
• Concentration: 0.2mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cytoskeleton Markers
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: Basic calponin, calponin 1, basic, smooth muscle, Calponin H1, smooth muscle, calponins, basic, Sm-Calp, SMCCcalponin-1
• Gene Symbols: CNN1
• Isotype: IgG
• Purification Method: Protein A or G purified
• Test Specificity: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

• Clone: CNN1/832 + CALP
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Rat
• Gene Accession No.: P51911
• Gene ID (Entrez): 1264
• Immunogen: Recombinant full-length human CNN1 protein (CNN1/832); Crude human uterus extract (CALP)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 34 kDa

For Research Use Only