CD117/c-kit Antibody (KIT/982), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2447610.02MG

Catalog No. NBP24476100

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence

CD117/c-kit Monoclonal specifically detects CD117/c-kit in Human samples. It is validated for Western Blot, Protein Array.

Specifications

• Antigen: CD117/c-kit
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 2 - 4 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Alias: CD117, CD117 antigen, ckit, C-Kit, EC 2.7.10, EC 2.7.10.1, PBT, piebald trait, Proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, SCFRmast/stem cell growth factor receptor, soluble KIT variant 1, Tyrosine-protein kinase Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb recognizes a protein of 145kDa, identified as CD117/p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Frozen), SDS-Page, Immunofluorescence
• Clone: KIT/982
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: KIT
• Immunogen: Recombinant human CD117 fragment around aa100-300
• Quantity: 0.02 mg
• Research Discipline: Cancer, Cancer Stem Cells, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Protein Kinase, Signal Transduction, Stem Cell Markers, Tyrosine Kinases
• Gene ID (Entrez): 3815
• Target Species: Human
• Form: Purified

For Research Use Only