CD3 Mouse anti-Human, APC-R700, Clone: UCHT1, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 565119
The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε .
This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor (eg, 730/45-nm filter).
|CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon|
|Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor|
|Aqueous buffered solution containing BSA, protein stabilizer, glycerol, and ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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