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CD45 Mouse, APC-R700, Clone: D058-1283, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 565182

Catalog No. BDB565182

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D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes, developed using Rhesus peripheral whole blood as immunogen. It does not cross react with human leucocytes. This antibody reacts with Baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern as seen with CD45 binding to the Leukocyte Common Antigen on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a band of approximately 180-200 kDa molecular weight .

This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45-nm filter).



Pan Leukocyte, NHP-specific; PTPRC; CD45R; LCA; L-CA; Leukocyte Common Ag
Affinity Purified
Rhesus peripheral whole blood
Affinity Chromatography
Baboon, Cynomolgus, Rhesus
Flow Cytometry
Aqueous buffered solution containing BSA, protein stabilizer, glycerol and ≤0.09% sodium azide.
IgG1, κ
50 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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