CD99 Antibody (12E7 + MIC2/877), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2446970.1ML

Catalog No. NBP24469701

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

CD99 Monoclonal specifically detects CD99 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: CD99
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Flow Cytometry 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1:100-1:200, Immunofluorescence 1:100-1:200
• Gene Accession No.: P14209, P14209
• Gene Symbols: CD99
• Immunogen: Human acute lymphocytic leukemia T-cells (12E7); Recombinant human MIC2 protein (MIC2/877)
• Quantity: 0.1 mL
• Research Discipline: Immunology
• Gene ID (Entrez): 4267
• Target Species: Human
• Form: Supernatant

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: 12E7 + MIC2/877
• Conjugate: Unconjugated
• Formulation: No buffer with 0.05% Sodium Azide
• Gene Alias: 12E7, antigen identified by monoclonal antibodies 12E7, F21 and O13, CD99 antigenY homolog, CD99 molecule, E2 antigen, HBA71, MIC2 (monoclonal 12E7), MIC2Y, MSK5X, Protein MIC2, surface antigen MIC2, T-cell surface glycoprotein E2
• Host Species: Mouse
• Purification Method: Tissue culture supernatant
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewings sarcoma cells and primitive peripheral neuroectodermal tumors.
• Content And Storage: Store at 4C.
• Isotype: IgG

For Research Use Only