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Mouse anti-CD99, Clone: SPM586, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2446930.1MG

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Catalog No. NBP24469301

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Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

CD99 Monoclonal antibody specifically detects CD99 in Human, Rat samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.


10mM PBS with 0.05% Sodium Azide
12E7, antigen identified by monoclonal antibodies 12E7, F21 and O13, CD99 antigenY homolog, CD99 molecule, E2 antigen, HBA71, MIC2 (monoclonal 12E7), MIC2Y, MSK5X, Protein MIC2, surface antigen MIC2, T-cell surface glycoprotein E2
IgM Kappa
0.1 mg
Human, Rat
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Flow Cytometry 5 - 10ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1 - 2ug/ml, Immunofluorescence 0.5 - 1.0ug/ml, CyTOF-ready
Purified E-rosette forming cells from human peripheral blood lymphocytes
Protein A or G purified
Store at 4C.
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
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