CD99 Antibody (SPM586), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24469300	Novus Biologicals NBP2446930.02MG	0.02 mg
NBP24469301	Novus Biologicals NBP2446930.1MG	0.1 mg
NBP24469302	Novus Biologicals NBP2446930.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

CD99 Monoclonal specifically detects CD99 in Human, Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: CD99
• Concentration: 0.2mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Immunology
• Formulation: 10mM PBS with 0.05% Sodium Azide
• Gene ID (Entrez): 4267
• Immunogen: Purified E-rosette forming cells from human peripheral blood lymphocytes
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: SPM586
• Dilution: Flow Cytometry 5 - 10ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1 - 2ug/ml, Immunofluorescence 0.5 - 1.0ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Rat
• Gene Alias: 12E7, antigen identified by monoclonal antibodies 12E7, F21 and O13, CD99 antigenY homolog, CD99 molecule, E2 antigen, HBA71, MIC2 (monoclonal 12E7), MIC2Y, MSK5X, Protein MIC2, surface antigen MIC2, T-cell surface glycoprotein E2
• Gene Symbols: CD99
• Isotype: IgM κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

For Research Use Only