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Mouse anti-Cyclin B1, Clone: CCNB1/1098, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2446870.2MG

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Catalog No. NBP24468702

This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Specifications

Specifications

Cyclin B1
CCNB1/1098
Unconjugated
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Purified
CCNB1
Recombinant human full-length CCNB1 protein
Protein A or G purified
RUO
Store at 4C.
Monoclonal
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
0.2mg/mL
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
P14635
CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1
Mouse
IgG1 Kappa
0.2 mg
Cancer, Cell Biology, Cell Cycle and Replication, Mitotic Regulators, Tumor Suppressors
Primary
891
Human, Mouse
Documents
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