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Mouse anti-Cytokeratin 5/8, Clone: SPM268, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2447030.1MG

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Catalog No. NBP24470301

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Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytokeratin 5/8 Monoclonal antibody specifically detects Cytokeratin 5/8 in Human, Mouse, Rat, Porcine, Canine, Ferret, Hamster, Primate, Sheep, Chicken (Negative), Xenopus (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence.


Cytokeratin 5/8
10mM PBS (pH 7.4) and 0.05% BSA with 0.05% Sodium Azide
Cytoskeletal preparation from HeLa cells
Protein A or G purified
Human, Xenopus (Negative), Mouse, Rat, Porcine, Canine, Ferret, Hamster, Primate, Sheep, Chicken (Negative)
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
P13647, P05787
cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5
IgG1 Kappa
0.1 mg
Store at 4C.
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
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