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Mouse anti-EMI1, Clone: EMI1/1176, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2443440.2MG

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Catalog No. NBP24434402

This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Specifications

Specifications

EMI1
EMI1/1176
Unconjugated
1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Purified
FBXO5
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
56 kDa
0.2 mg
Store at 4C.
Monoclonal
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
0.2mg/mL
Western Blot 1 - 2 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Q9UKT4
EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31
Mouse
IgG2a Kappa
Protein A or G purified
RUO
Primary
26271
Human
Documents
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