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Mouse anti-Factor VIII, Clone: RFF-VIIIC/10, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus Biologicals NBP1410210.1MG

Catalog No. NBP14102101

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Factor VIII Monoclonal antibody specifically detects Factor VIII in Human samples. It is validated for Western Blot, ELISA, Radioimmunoassay.


Factor VIII
PBS (pH 7.4) with 0.09% Sodium Azide
Affinity purified human Factor VIII
Protein G purified
ELISA, Radioimmune Assays, Western Blot
1.0 mg/mL
Western Blot 1:100, ELISA 1:100-1:2000, Radioimmunoassay 1:100-1:2000
AHF, Antihemophilic factor, coagulation factor VIII, procoagulant component, coagulation factor VIIIc, DXS1253E, F8Ccoagulation factor VIII, factor VIII F8B, FVIII, HEMAF8B, Procoagulant component
0.1 mg
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
This antibody recognises human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Clone RFF-VIIIC/10 recognises an epitope within the non-functional, middle domain of full-length Factor VIII.
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